Plant Defense Genes: Nicotiana benthamiana
Chakravarthy et al, 2010. Identification of Nicotiana benthamiana genes involved in PAMP-triggered immunity (PMID:pending)
Genes comprising the immunity-induced gene collection identified in tomato (Excel)
Nicotiana benthamiana genes implicated in PTI - BLAST analysis (Excel)
Model summarizing the possible roles of genes linked to PAMP-triggered immunity
(click to see larger image)
Nicotiana benthamiana genes compromising PTI when silenced
N. benth
gene silenced |
Category |
source |
Percentage of plants showing compromised PTI with the indicated inducer/challenger combination |
Pf/DC |
Pp/DC |
Pf/Q1-1 |
Agro/Ptab |
TRV2 |
- |
- |
0% |
0% |
0% |
0% |
BAK1 |
signaling |
VIGS library |
53% |
22% |
44% |
3% |
FLS2 |
PRR |
VIGS library |
75% |
48% |
66% |
5% |
ALDH |
secondary metabolism |
VIGS library |
8% |
4% |
21% |
8% |
Cathepsin B |
protease |
VIGS library |
17% |
17% |
33% |
8% |
Cyclophilin |
protein folding |
VIGS library |
33% |
26% |
44% |
4% |
Cytochrome C |
secondary metabolism |
VIGS library |
32% |
8% |
32% |
4% |
Drm-3 |
hormone signalling |
VIGS library |
45% |
11% |
17% |
6% |
HCBT |
secondary metabolism |
VIGS library |
20% |
24% |
16% |
8% |
Plastocyanin |
secondary metabolism |
VIGS library |
29% |
13% |
38% |
13% |
Alternative oxidase |
energy metabolism |
immunity induced |
20% |
13% |
40% |
7% |
Anionic peroxidase |
cell wall protein |
|
42% |
47% |
47% |
5% |
CA4H |
secondary metabolism |
|
43% |
21% |
50% |
29% |
EDS1 |
defense signaling |
|
27% |
40% |
27% |
7% |
proteasome 26S subunit |
protein stability |
|
36% |
27% |
0% |
9% |
Trunsducin |
signaling |
|
8% |
8% |
15% |
46% |
Ubiquitin activatinng enzyme |
protein stability |
|
12% |
18% |
29% |
25% |
Percentages in bold represent those combinations where PTI was compromised as determined by pairwise comparisons of the TRV2 control with each of the candidate genes with a Fisher’sexact test (α = 0.05).
Abbreviations: HCBT = Anthranilate N hydroxycinnamoyl/benzoyltransferase, ALDH = Aldehyde dehydrogenase, Agro = A.
tumefaciens, DC = Pst DC3000, Pf = P. fluorescens, Pp = P. putida, Ptab = P. s. pv. tabaci, Q1-1 = Pst DC3000 deltahopQ1-1.
Extent of cell death in Nicotiana benthamiana caused by inoculation with different challengers
N. benth
gene silenced |
Extent of cell death upon
inoculation with Pto DC3000 |
Extent of cell death upon
inoculation with Pto DC3000 deltahopQ1-1 |
Full |
Partial |
None |
Full |
Partial |
None |
TRV2 |
8 |
3 |
1 |
2 |
1 |
5 |
BAK1 |
1 |
3 |
4 |
4 |
4 |
0 |
FLS2 |
8 |
3 |
1 |
2 |
1 |
5 |
ALDH |
7 |
3 |
2 |
3 |
2 |
3 |
Cathepsin B |
5 |
4 |
3 |
2 |
3 |
3 |
Cyclophilin |
4 |
5 |
3 |
2 |
4 |
1 |
Cytochrome C |
6 |
4 |
2 |
2 |
2 |
4 |
Drm-3 |
7 |
2 |
3 |
0 |
2 |
6 |
HCBT |
2 |
5 |
5 |
0 |
4 |
4 |
Plastocyanin |
4 |
6 |
2 |
2 |
2 |
4 |
|
