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Plant Defense Gene Characterization

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Plant Defense Genes: Nicotiana benthamiana

Chakravarthy et al, 2010. Identification of Nicotiana benthamiana genes involved in PAMP-triggered immunity (PMID:pending)

Genes comprising the immunity-induced gene collection identified in tomato (Excel)
Nicotiana benthamiana genes implicated in PTI - BLAST analysis (Excel)

Model summarizing the possible roles of genes linked to PAMP-triggered immunity
(click to see larger image)


PTI-response genes

Nicotiana benthamiana genes compromising PTI when silenced

N. benth
gene silenced
Category
source
Percentage of plants showing compromised PTI with the indicated inducer/challenger combination
Pf/DC
Pp/DC
Pf/Q1-1
Agro/Ptab
TRV2
-
-
0%
0%
0%
0%
BAK1
signaling
VIGS library
53%
22%
44%
3%
FLS2
PRR
VIGS library
75%
48%
66%
5%
ALDH
secondary metabolism
VIGS library
8%
4%
21%
8%
Cathepsin B
protease
VIGS library
17%
17%
33%
8%
Cyclophilin
protein folding
VIGS library
33%
26%
44%
4%
Cytochrome C
secondary metabolism
VIGS library
32%
8%
32%
4%
Drm-3
hormone signalling
VIGS library
45%
11%
17%
6%
HCBT
secondary metabolism
VIGS library
20%
24%
16%
8%
Plastocyanin
secondary metabolism
VIGS library
29%
13%
38%
13%
Alternative oxidase
energy metabolism
immunity induced
20%
13%
40%
7%
Anionic peroxidase
cell wall protein
immunity induced
42%
47%
47%
5%
CA4H
secondary metabolism
immunity induced
43%
21%
50%
29%
EDS1
defense signaling
immunity induced
27%
40%
27%
7%
proteasome 26S subunit
protein stability
immunity induced
36%
27%
0%
9%
Trunsducin
signaling
immunity induced
8%
8%
15%
46%
Ubiquitin activatinng enzyme
protein stability
immunity induced
12%
18%
29%
25%
Percentages in bold represent those combinations where PTI was compromised as determined by pairwise comparisons of the TRV2 control with each of the candidate genes with a Fisher’sexact test (α = 0.05).
Abbreviations: HCBT = Anthranilate N hydroxycinnamoyl/benzoyltransferase, ALDH = Aldehyde dehydrogenase, Agro = A.
tumefaciens, DC = Pst DC3000, Pf = P. fluorescens, Pp = P. putida, Ptab = P. s. pv. tabaci, Q1-1 = Pst DC3000 deltahopQ1-1.



Extent of cell death in Nicotiana benthamiana caused by inoculation with different challengers

N. benth
gene silenced
Extent of cell death upon
inoculation with Pto DC3000
Extent of cell death upon
inoculation with Pto DC3000 deltahopQ1-1
Full
Partial
None
Full
Partial
None
TRV2
8
3
1
2
1
5
BAK1
1
3
4
4
4
0
FLS2
8
3
1
2
1
5
ALDH
7
3
2
3
2
3
Cathepsin B
5
4
3
2
3
3
Cyclophilin
4
5
3
2
4
1
Cytochrome C
6
4
2
2
2
4
Drm-3
7
2
3
0
2
6
HCBT
2
5
5
0
4
4
Plastocyanin
4
6
2
2
2
4

 

 


Magdalen Lindeberg
PPI Project Coordinator
Plant Pathology and Plant-Microbe Biology
Cornell University
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