ࡱ > ` ev bjbjss 3 en < , ħ 4 ) L P P P P + + + $ h C T + + + + + P P Ѵ Ѵ Ѵ + P P Ѵ + Ѵ Ѵ P W# j ķ d D2 0 ) ̷ , S h l Ѵ + + + + + + ) + + + + $ . < For more information see:
HYPERLINK "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed" Ferreira, A. et al., 2006. Whole-genome expression profiling defines the HrpL regulon of Pseudomonas syringae pv tomato DC3000 allows de novo reconstruction of the Hrp cis element, and identifies novel co-regulated genes. MPMI 19:1167-1179.
Supplementary Table S2. Computationally identified Hrp promoters in P. syringae pv. tomato DC3000 and their associated downstream targets. Each row corresponds to a Hrp promoter identified by scanning the complete DC3000 genome with the HMM2 and WM2 models (see text for details), so genes and/or operons can appear more than once if there are multiple putative promoter hits. Entries are sorted in descending order according to their HMM2 scores. Additional entries at the bottom of the table are included for which there is experimental evidence for HrpL-dependent expression under hrpL overexpression, but for which no discernable Hrp promoter is evident.OPERONEVIDSTARTSTOPSGEODISTHMM2WM2GCPROTEIN ANNOTATIONS588CM648424648456-I/C-21226.17.244.6HopH14599-4597FM-CM51926135192645-I/C-6224.27.142.4T3SS chaperone ShcS1 HopS1::ISPssy4331CM48810974881129+I/C-7023.67.048.9HopE1 877CM949826949858-E/N-6023.36.152.2HopQ1-1 2105FCR22798832279915+I/C-14322.86.359.8ApbE-family protein4753053844795384511+E/N-470622.56.255.4hypothetical protein4001FCM45152964515328-I/C-6822.36.645.7AvrPto147330R53617075361739-I/C-8522.26.852.2hypothetical protein3087FCM34701853470217-I/C-8621.26.753.3HopAB2 (AvrPtoB)883FCM954203954235+I/C-20520.76.744.6HopR1 1378FCM15196661519698+I/C-5920.75.856.5membrane-bound lytic murein transglycosylase D, putative1381-1382-1383-1384-1385-1386CM-CM-M-M-M-M15242041524236+I/C-7720.65.748.9T3SS components HrpA1 - HrpZ1 - HrpB - HrcJ - HrpD - HrpE589FCM649735649767-E/N-6220.47.043.5HopC1876FCM946154946186+I/C-6920.46.255.4HopD11387-1388-1389-1390-1391CN-M-M-N-M15281841528216+I/C-6220.46.354.4T3SS components HrpF - HrpG - HrcC - HrpT - HrpV1403-1402-1401-1400-1399-1398-1397-1396-1395-1394-1393-1392CM-M-M-0-M-M-M-M-M-N-0-015426211542653-I/C-5920.45.855.4T3SS components HrpJ - HrcV - HrpQ - HrcN - HrpO - HrpP - HrcQa - HrcQb - HrcR - HrcS - HrcT - HrcU5617*NR939413939445-E/N-9620.26.845.7conserved hypothetical protein (pseudogene)5053057514755751507+E/C-48719.85.556.5hypothetical protein2678-2679FCM-N29738252973857+I/C-12119.66.947.8HopP1 - hypothetical protein834-(835-836-837-838)CR-C0-C0-0-0905339905371+I/C-23619.26.350.0alcohol dehydrogenase, zinc-containing - ribD C-terminal domain protein - conserved domain protein - hypothetical protein - major facilitator family transporter8730941100941132+I/C-91919.25.944.6amidinotransferase family protein503-(502-501)M-FC0-0550602550634-I/C-6518.86.339.1T3SS chaperone ShcF - HopF2 - HopU147180R53443755344407-I/C-19618.85.951.1HopAA1-21372FCM15076521507684+I/C-19418.75.955.4HopAA1-14589-4588C0-CN51861235186155-I/C-6118.75.357.6T3SS chaperone ShcS2 HopS2A0005FM65956627-I/C-6618.45.848.9HopAM1-21022FCM11163781116410-I/C-6618.45.848.9HopAM1-11369-1370FM-FCM15048861504918+I/C-6318.46.141.3conserved effector locus protein - HopN15240R572473572505+I/C-10118.36.352.2peptidase, M20 - M25 - M40 familyA0012FM1610316135-I/C-6718.15.840.2HopX11374-1375-(1376)CM15108811510913+I/C-8917.95.045.7T3SS chaperone ShcM - HopM1 T3SS chaperone ShcE5353-5354CM-FCM60857566085788+I/C-5917.56.053.3T3SS chaperone ShcA - HopA15620*-15680-CR17314211731453-E/N-28817.05.843.5hypothetic protein identified by sequence similarity - HopAF12856032121513212183-I/C-163416.85.148.9site-specific recombinase, phage integrase family4699053280195328051-I/C-85416.85.635.9non-ribosomal peptide synthetase, terminal component4776FCR54181975418229-I/C-5616.56.052.2HopI1A0017-A0018-A0019M-M-M1965819690+I/C-6116.45.756.5T3SS chaperone ShcO1 - HopO1-1 - HopT1-11405-1406CM15434161543448+I/C-6416.46.351.1HrpK1 - HopB161FCM8244782479+I/C-5416.35.453.3HopY15622*-1408-14090-FN-M(i)15483891548421+I/C-53116.15.547.8hypothetical protein - hypothetical protein hypothetical proteinB00078*-B0005P-MR68946926-I/C-82016.06.445.7unannotated protein - phosphoesterase family protein 4724-4725-4726#N53500345350066+I/C-65516.05.747.8HopD (interruption-N) - IS52, transposase - Hop D (interruption-C)[4579]051761335176165+E/C68115.95.457.6peptide chain release factor 3852FCM921879921911-I/C-21915.56.147.8HopAJ11373FCM15107851510817-I/C-15315.46.155.4HrpW11377C015195701519602-I/C-9915.25.750.0AvrE14101FCM46211294621161+I/C-9515.15.954.4HopAK15616*-474-473F0R-0522444522476-I/C-30914.76.250.0conserved hypothetical protein - HopAS1 (interruption-N) - HopAS1 (interruption-C)4344049051894905221+E/C-145614.65.064.1insecticidal toxin protein, putative4727CM53552245355256-I/C-9014.45.745.7HopG14464050242155024247+E/N-131614.35.357.6conserved hypothetical protein4276048201364820168-E/N-212713.94.756.5transcriptional regulator, LysR family4722 (4721 4720)FC053485785348610-I/C-8613.85.147.8HopAO1 T3SS chaperone ShcV HopV14370488341488373-E/N-664513.35.348.9dihydrofolate reductase44CR6150461536-E/N-11313.25.451.1HopK1370-(371)0-FC0404752404784+I/C-11413.25.552.2MATE efflux family protein - indoleacetate-lysine ligase (iaaL)4703CN53306885330720+I/C-11413.25.452.2HopAQ11398-1397-1396-1395-1394-1393-1392M-M-M-M-M-N-0-015368741536906-E/C-87013.15.457.6T3SS components HrpP - HrcQa - HrcQb - HrcR - HrcS - HrcT - HrcU[1707]018768801876912-E/C110412.95.360.9L-sorbosone dehydrogenase1841020084052008437+E/N-88712.94.453.3threonine aldolase, low-specificity1996-1995-19940-0-N-021820882182120-E/C-12012.9NA66.3copper-translocating P-type ATPase - cytochrome oxidase maturation protein, cbb3-type - membrane protein, putative1370FCM15052191505251+E/C-22212.84.457.6HopN12346026000722600104+E/N-106112.74.468.54-hydroxyphenylpyruvate dioxygenase, putative1869020445532044585-E/C-124312.55.155.4conserved hypothetical protein5200059084185908450+E/N-954512.54.965.2autotransporter, putative1645018023041802336+E/C-72212.34.862.0transcriptional regulator, SlyA/MarR family3019033934103393442+E/C-57912.34.856.5oxidoreductase, Gfo - Idh - MocA family5522062891946289226+E/N-24912.35.055.4conserved hypothetical protein[1268]013919651391997-E/C22412.24.667.4valyl-tRNA synthetase4721053467585346790-I/C-6012.25.643.5hypothetical protein4205047332224733254+E/N-396012.04.868.5transcriptional regulator, GntR family[B0069]N5906059092+E/C60211.84.759.8traY protein5089057905105790542+E/N-97711.84.855.4conserved hypothetical protein5619* - 901C0981177981209+I/C-32411.75.239.1putative T3SS chaperone - candidate HopAG1::ISPssy[4248]047867174786749+E/C76511.75.050.03-hydroxyacyl-CoA-acyl carrier protein transferase2696029902462990278-I/C-24111.64.448.9mutT - nudix family protein3489-3488M(i)-M39396583939690-E/C-31711.64.459.8sugar ABC transporter, ATP-binding protein - sugar ABC transporter, permease protein 932010148261014858-E/N-395511.54.955.4sugar ABC transporter, permease protein5057057616095761641-I/C-31611.54.664.1dihydroxy-acid dehydratase4920055690895569121+I/C-26911.45.151.1transcriptional regulator, LysR family1190142796142828+E/C-17911.34.951.1peptidase, M16 family224-223-222-2210247050247082-E/C-68111.34.962.0diaminopimelate epimerase - conserved hypothetical protein - integrase - recombinase XerC - HAD-superfamily hydrolase2130023043282304360-E/C-22011.34.851.1DNA-binding response regulator, LuxR family3984044895524489584+I/N-143611.35.262.0cold shock domain family protein[4005]045190004519032+E/C41811.34.755.4hypothetical protein1213013298601329892-E/N-78311.24.554.4transcriptional regulator, AraC family[1842]020121072012139+E/C166711.24.554.4alanyl-tRNA synthetase2145-2144-2143-2142-2141-2140-2139-2138023548452354877-E/N-2578411.25.151.1iron-regulated membrane protein, putative - conserved hypothetical protein - conserved hypothetical protein - conserved hypothetical protein - cation ABC transporter, periplasmic cation-binding protein - cation ABC transporter, ATP-binding protein - cation ABC transporter, permease protein - ABC transporter, periplasmic substrate-binding protein, putative3721041996034199635+E/C-122011.04.662.0enoyl-(acyl-carrier-protein) reductase[2362]026159522615984+E/C51310.94.860.9methionine aminopeptidase, type I3270N36935983693630+E/N-244210.94.257.6conserved hypothetical protein5623* -4732MR53555305355562+I/N-432410.94.555.4HopH::IsPsy4 - HopQ1-26650714155714187+E/N-437710.74.468.5ROK family protein3000325510325542+I/C-25510.64.253.3succinate-semialdehyde dehydrogenase2107022872582287290-E/N-393110.64.156.5glycerophosphoryl diester phosphodiesterase, putative4157-4158046844624684494+E/N-26410.65.159.8dihydroorotase, homodimeric type - ribonuclease T4976056497535649785-E/N-131210.65.162.0thiamin biosynthesis protein ThiC[4996]056734525673484+E/C16310.64.962.0conserved domain protein2431026831812683213+E/C-96910.54.844.6conserved hypothetical protein3641040997254099757+E/C-63910.54.064.1endoribonuclease L-PSP family protein4516.1050859285085960+E/C-25510.54.654.4tmRNA, putative [1][2151]023582862358318-E/C119410.44.460.9TonB-dependent siderophore receptor, putative4381049435334943565-E/N-35310.44.865.2conserved hypothetical protein5045057521845752216-E/N-638810.44.966.3twitching motility protein2162023777122377744-E/C-53310.34.355.4hypothetical protein4294-4295048392094839241+I/C-10710.34.356.5chaperone protein HscC - DnaJ domain protein[4955]056166705616702+E/C88110.34.357.6rhodanese domain protein - phosphatidylserine decarboxylase5396061252426125274+I/N-320210.34.266.3dTDP-glucose 4,6-dehydratase4507050744815074513+E/N-411910.24.964.1DNA repair protein RecN1621-1620-1619017799691780001-E/N-190210.15.063.0conserved hypothetical protein - conserved hypothetical protein - hypothetical protein[2014]N22017762201808-E/C13510.14.363.0aerotaxis receptor5150-5151-5152058592725859304+E/N-172910.14.668.5ubiquinone - menaquinone biosynthesis methlytransferase UbiE - conserved hypothetical protein - ubiquinone biosynthesis protein UbiB5353-5354CM60854636085495+E/C-35210.14.557.6T3SS chaperone ShcA HopA1371FC0406209406241+E/C-11410.04.854.4indoleacetate-lysine ligase (iaaL)6750731309731341-I/C-109.64.4/4.553.3arylesterase1209-1208013259531325985-I/C -1779.13.8/4.347.8RNA polymerase sigma-70 factor regulatory protein, putative4691 C053052205305252+I/C-5838.73.8/4.447.8HopAD11278014051121405144-I/C-447.74.2/4.956.5sensory box protein/response regulator1708-1709-1710-1711-1712-1713-1714-1715-1716-1717018783141878346+I/C-1277.13.9/3.864.1cobO operon: cob(I)yrinic acid a,c-diamide adenosyltransferase nitroreductase family protein cobD cobC cobQ cobP cobT - alpha-ribazole-5'-phosphate phosphatase, putative - cobS2497-2498N27626792762711-I/C-546.94.1/4.646.7hypothetical protein hypothetical protein4071045773284577360-I/C-396.43.9/4.252.2hypothetical protein2936033008623300894-I/C-526.14.5/3.846.7LamB/YscF family protein2525027879082787940+E/N-341<6.04.7/4.159.8peptide ABC transporter, permease protein2790031082333108265+E/C<6.04.0/4.238.0hypothetical protein4964056301885630220-I/C-41<6.04.1/4.053.3conserved hypothetical protein4010445291445323-I/C-233<6.03.9/3.960.9iron-sulfur cluster-binding protein, Rieske family1672018435671843599+I/C-47<6.04.0/3.963.0DNA-binding response regulator5618*N922923922955-I/C<6.04.1/4.546.7HopAT1 (frameshift)1407CNNO HRP PROMOTERpseudogene not annotated as CDS4680C0NO HRP PROMOTERcoronafacic acid synthetase, ligase component (cfl)4681-4682-4683-4684-4685-4686-4687-4688-4689-4690F-N-N-N-N-F-N-N-N-NNO HRP PROMOTERcoronafacic acid (cfa) synthetase components cfa1 through cfa7 - hypothetical protein - cfa8- cfa9Entries are color coded as follows:
BLUE: experimental evidence for HrpL-dependent expression both under conditions of hrpL deletion in Hrp minimal medium as examined here, and hrpL overexpression in rich medium, as previously reported ADDIN EN.CITE Fouts20025167516717Fouts, D EAbramovitch, R BAlfano, J RBaldo, A MBuell, C RCartinhour, SChatterjee, A KD'Ascenzo, MGwinn, M LLazarowitz, S GLin, N-CMartin, G BRehm, A HSchneider, D Jvan Dijk, KTang, XCollmer, AProc. Natl. Acad. Sci. USAProc. Natl. Acad. Sci. USA2275-228099h/r2002C:\Documents and Settings\arc2.CALSNET\My Documents\A\5167.pdfChang20056185618517Chang, J. H.Urbach, J. M.Law, T. F.Arnold, L. W.Hu, A.Gombar, S.Grant, S. R.Ausubel, F. M.Dangl, J. L.Departments of Biology CB#3280 and Microbiology and Immunology CB#7290, and Curriculum in Genetics, Carolina Center for Genome Sciences, University of North Carolina, Chapel Hill, NC 27599.Proc. Natl. Acad. Sci. U S AProc. Natl. Acad. Sci. U S A2549-25541027h/t/z2005Feb 1515701698C:\Documents and Settings\arc2.CALSNET\My Documents\A\6185.pdfC:\Documents and Settings\arc2.CALSNET\My Documents\A\6185-supporting text.docC:\Documents and Settings\arc2.CALSNET\My Documents\A\6185-table2.xlsC:\Documents and Settings\arc2.CALSNET\My Documents\A\6185-table3.xlsC:\Documents and Settings\arc2.CALSNET\My Documents\A\6185-table4.xlsC:\Documents and Settings\arc2.CALSNET\My Documents\A\6185-table5.xls(Chang et al., 2005; Fouts et al., 2002).
GREEN: experimental evidence for HrpL-dependent expression under conditions of hrpL deletion in Hrp minimal medium as examined here, but not under hrpL overexpression in rich medium, as previously reported ADDIN EN.CITE Fouts20025167516717Fouts, D EAbramovitch, R BAlfano, J RBaldo, A MBuell, C RCartinhour, SChatterjee, A KD'Ascenzo, MGwinn, M LLazarowitz, S GLin, N-CMartin, G BRehm, A HSchneider, D Jvan Dijk, KTang, XCollmer, AProc. Natl. Acad. Sci. USAProc. Natl. Acad. Sci. USA2275-228099h/r2002C:\Documents and Settings\arc2.CALSNET\My Documents\A\5167.pdfChang20056185618517Chang, J. H.Urbach, J. M.Law, T. F.Arnold, L. W.Hu, A.Gombar, S.Grant, S. R.Ausubel, F. M.Dangl, J. L.Departments of Biology CB#3280 and Microbiology and Immunology CB#7290, and Curriculum in Genetics, Carolina Center for Genome Sciences, University of North Carolina, Chapel Hill, NC 27599.Proc. Natl. Acad. Sci. U S AProc. Natl. Acad. Sci. U S A2549-25541027h/t/z2005Feb 1515701698C:\Documents and Settings\arc2.CALSNET\My Documents\A\6185.pdfC:\Documents and Settings\arc2.CALSNET\My Documents\A\6185-supporting text.docC:\Documents and Settings\arc2.CALSNET\My Documents\A\6185-table2.xlsC:\Documents and Settings\arc2.CALSNET\My Documents\A\6185-table3.xlsC:\Documents and Settings\arc2.CALSNET\My Documents\A\6185-table4.xlsC:\Documents and Settings\arc2.CALSNET\My Documents\A\6185-table5.xls(Chang et al., 2005; Fouts et al., 2002).
ORANGE: experimental evidence for HrpL-dependent expression under hrpL overexpression in rich medium, as reported ADDIN EN.CITE Fouts20025167516717Fouts, D EAbramovitch, R BAlfano, J RBaldo, A MBuell, C RCartinhour, SChatterjee, A KD'Ascenzo, MGwinn, M LLazarowitz, S GLin, N-CMartin, G BRehm, A HSchneider, D Jvan Dijk, KTang, XCollmer, AProc. Natl. Acad. Sci. USAProc. Natl. Acad. Sci. USA2275-228099h/r2002C:\Documents and Settings\arc2.CALSNET\My Documents\A\5167.pdfChang20056185618517Chang, J. H.Urbach, J. M.Law, T. F.Arnold, L. W.Hu, A.Gombar, S.Grant, S. R.Ausubel, F. M.Dangl, J. L.Departments of Biology CB#3280 and Microbiology and Immunology CB#7290, and Curriculum in Genetics, Carolina Center for Genome Sciences, University of North Carolina, Chapel Hill, NC 27599.Proc. Natl. Acad. Sci. U S AProc. Natl. Acad. Sci. U S A2549-25541027h/t/z2005Feb 1515701698C:\Documents and Settings\arc2.CALSNET\My Documents\A\6185.pdfC:\Documents and Settings\arc2.CALSNET\My Documents\A\6185-supporting text.docC:\Documents and Settings\arc2.CALSNET\My Documents\A\6185-table2.xlsC:\Documents and Settings\arc2.CALSNET\My Documents\A\6185-table3.xlsC:\Documents and Settings\arc2.CALSNET\My Documents\A\6185-table4.xlsC:\Documents and Settings\arc2.CALSNET\My Documents\A\6185-table5.xls(Chang et al., 2005; Fouts et al., 2002), but not under conditions of hrpL deletion in Hrp minimal medium as examined here.
GRAY: no experimental evidence for HrpL-dependent expression
For each Hrp promoter, the following items are identified:
OPERON: the predicted downstream target(s) of the promoter. Operon members enclosed in parentheses are assumed to be part of the stated operon because of experimental evidence, but which were not identified so by our computational operon prediction method. Operon members enclosed in square brackets are not considered to be actual downstream targets but are included for the sake of completeness; these represent genes in which the predicted Hrp promoter is embedded, with no plausible downstream target that can be identified (i.e., no subsequent genes in a predicted operon which could be controlled by the promoter).
NOTES:
* PSPTO numbers annotated with an asterisk refer to new gene calls that we are making based on this work. These new PSPTO identifiers are included in the updated GenBank file we are submitting in conjunction with this paper.
# entry 4724-4725-4726 is presumed vestigial, although the promoter may indeed be functional; 4725 is a transposase which appears to have inserted into, and disrupted, a copy of HopD1, which is composed of the fragments in 4724 and 4726.
EVID: summary of experimental evidence for HrpL-dependent expression, listing an entry for each member of the predicted operon, respectively; if only one entry is listed for a polycistronic operon, that evidence label applies to every member of the operon
M = exhibited differential expression (up-regulation) on our Microarray
M(i) = = exhibited differential expression (up-regulation) on our Microarray, but just missed our spot intensity cutoff of 4000 units; reducing that cutoff to 3900 units included two extra spots, associated with PSPTO1409 and PSPTO3489. For further evidence of expression of PSPTO1409, see Petnicki-Ocwiega et al. (2005).
R = exhibited differential expression (up-regulation) in our RT-PCR assays
C = listed in Chang et al. (2005) Table 2 with nonzero reads in functional screen for DC3000
F = listed in Fouts et al. (2002), Table 2
0 = on our Microarray, but did not exhibit differential expression
N = not on our Microarray
P = evidence for existence of peptide fragment via proteomics
START: start coordinate of Hrp promoter
STOP: stop coordinate of Hrp promoter
S: strand of Hrp promoter
GEO: geometry of the Hrp promoter hit with respect to the local genome
I = Intergenic (start of Hrp promoter is located between two predicted genes)
E = Embedded (start of Hrp promoter is located within a predicted coding region)
C = Coding: if hit is Embedded, then Hrp promoter is oriented in the coding direction of the gene in which it is embedded; if hit is Intergenic, then next downstream gene is on the same strand as the Hrp promoter
N = Noncoding: if hit is Embedded, then Hrp promoter is oriented oppositely to the coding direction of the gene in which it is embedded; if hit is Intergenic, then next downstream gene is on the opposite strand of the Hrp promoter\
DIST: distance from the start of the Hrp promoter to the start of the predicted operon (NOTE: distance is calculated on the same strand as the Hrp promoter, even if the next downstream gene is on the opposite strand (e.g., Intergenic/Noncoding)); negative distances indicate the start of the Hrp promter is upstream of the start of the target operon; positive distances indicate the start of the Hrp promoter is downstream of the start of the gene; in this case, the Hrp promoter is embedded
HMM2: score of Hrp promoter within the Hidden Markov Model level 2 model
WM2: score of Hrp promoter within the Weight Matrix level 2 model (number of standard deviations above the mean)
GC: percentage of G+C nucleotides in a window around and including the Hrp promoter, extending 40 bases upstream of the start of the Hrp promoter and 20 bases downstream of the stop
PROTEIN ANNOTATIONS: GenBank annotations of the protein corresponding to each member of the predicted operon. Abbreviations in annotations: TT = Type III; prot = protein. T3SS substrate names are updated to reflect the new Hop nomenclature conventions (Lindeberg et al. 2005).
l m z _ a x y ³l\lL hxa3 B*CJ OJ QJ aJ ph h B*CJ OJ QJ aJ ph %hR hJ B*CJ OJ QJ aJ ph h{ hJ CJ OJ PJ QJ aJ #h{ h{ 6CJ OJ PJ QJ aJ h{ h{ CJ OJ PJ QJ aJ h{ h{ CJ OJ QJ aJ h{ h{ 5CJ OJ QJ aJ hOuG hOuG 0J
hOuG 0J hOuG hOuG 0J hOuG hOuG j hOuG hOuG UhOuG _ ` a
# & $$1$ 7$ 8$ H$ If a$gdJ $1$ 7$ 8$ H$ If gdJ 9 kd $$If l F2 d2 d2 4
l aN $If gd{ ev & : ; ? B I P R V [ ` d i o p z Ff $$1$ 7$ 8$ H$ If a$gdJ Ff $1$ 7$ 8$ H$ If gdJ
!
%
*
.
Ff $$1$ 7$ 8$ H$ If a$gdJ $1$ 7$ 8$ H$ If gdJ Ff
$If gdJ .
M N O S T yiiYII hA B*CJ OJ QJ aJ ph hL B*CJ OJ QJ aJ ph hw0 B*CJ OJ QJ aJ ph h B*CJ OJ QJ aJ ph hqc B*CJ OJ QJ aJ ph -hJ hJ B*CJ OJ QJ aJ mHph sHhi B*CJ OJ QJ aJ ph hxa3 B*CJ OJ QJ aJ ph hJ B*CJ OJ QJ aJ ph %hR hJ B*CJ OJ QJ aJ ph h| hJ CJ OJ QJ .
3
<
=
B
F
N
V
X
\
a
f
j
o
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# ' , 0 Ff# $$1$ 7$ 8$ H$ If a$gdJ Ff $1$ 7$ 8$ H$ If gdJ 0 5 J K P T \ d f j n s w | Ff- $$1$ 7$ 8$ H$ If a$gdJ Ff( $1$ 7$ 8$ H$ If gdJ / 0 N \ d l n r v { Ff7 $$1$ 7$ 8$ H$ If a$gdJ Ff2 $1$ 7$ 8$ H$ If gdJ
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