Sequencing
and annotation of the genome and two plasmids (pDC3000A and pDC3000B) was completed
by The Institute for Genome Research (TIGR) in March, 2003 and published in Buell
et al. 2003. Please note that these analyses do not reflect changes in the
annotation since its original deposition
General
features:
Feature | Chromosome | pDC3000A | pDC3000B |
Size (bp) | 6,397,126
bp | 73,661
bp | 67,473
bp | G+C
percentage | 58.4% | 55.1% | 56.1% |
Number
of ORFs | 5,615 | 71 | 77 |
Percent
Coding | 86.8% | 81.9% | 84.7% |
Average
ORF length | 988
nt | 849
nt | 742
nt | No.
ORFs encoding: | protein
with a known function | 3,402
(61%) | 52
(73%) | 46
(60%) | hypothetical
protein | 610
(11%) | 10
(14%) | 16
(21%) | conserved
hypothetical protein | 961
(17%) | 8
(11%) | 12
(16%) | protein
of unknown function | 642
(11%) | 1
(1%) | 3
(4%) |
Genome
publication and supporting information:
Buell,
et al. 2003. The complete sequence of the Arabidopsis and tomato
pathogen Pseudomonas syringae pv. tomato DC3000. Proc.
Natl. Acad. Sci 100:10181-10186. Supporting materials contain the following information:
supporting
Table 2 - DC3000 factors implicated in virulence and putative homologs in
pathogenic bacteria
supporting Table
3 - DC3000 genes disrupted by IS elements or phage genes supporting
Table 4 - IS elements present in DC3000 supporting
Table 5 - phage and bacteriocin regions in DC3000 supporting
Table 6 - genes with unknown function unique to DC3000 supporting
Fig 4 - functional classification of annotated ORFs in DC3000 supporting
Fig 5 - distribution of ORFs in paralogous families in DC3000 supporting
Fig 6 - virulence determinants in DC3000 and their distribution in other bacterial
pathogens
Additional
features of the original genome sequence and annotation are available
at the following sites:
TIGR-CMR Pseudomonas syringae DC3000
CAZy
- Database of carbohydrate active enzymes in DC3000 DNA
Structural Atlas - plots of structural features in the DC3000 chromosome generated
by the Center for Biological Sequence Analysis at the Technical University of
Denmark Transporter
Protein Analysis Database - TransportDB is a relational database describing
the predicted cytoplasmic membrane transport protein complement for organisms
whose complete genome sequence are available |