The
Pseudomonas-Plant Interaction Project originated with a proposal by
a group of investigators, headed by Dr. Alan Collmer at Cornell University,
to determine the genome sequence of P. syringae pv tomato
DC3000 and begin exploration of the functional genomics of its interaction
with the host plant tomato. P. syringae pv tomato DC3000
was chosen because it is the cause of bacterial speck on tomato and
Arabidopsis and because is is an important experimental model in molecular
plant pathology. Initial funding was provided by the NSF Plant Genome
Research Program grant DBI-0077622. (read
entire proposal)
Project
Objectives:
- complete
sequencing of the DC3000 genome
- systematic
identification of virulence factors
- cell biological
based analyses of DC3000-tomato interactions
- creation
of a Plant-Microbe Interaction Database
- genomics
oriented educational outreach
Achieving these
objectives has been greatly aided by collaboration with the USDA-ARS-CAB
modelling project at Cornell University
Investigators:
Alan
Collmer (PI) |
Cornell
University, Ithaca, NY [website] |
James
R. Alfano (co-PI) |
University
of Nebraska, Lincoln, NE [website]
|
C.
Robin Buell (co-PI)
|
The
Institute for Genomic Research, Rockville, MD [website] |
Samuel
W. Cartinhour (co-PI)
|
Cornell
University, Ithaca, NY [website] |
Arun
K. Chatterjee (co-PI)
|
University
of Missouri, Columbia, MO [website] |
Sondra
G. Lazarowitz (co-PI)
|
Cornell
University, Ithaca, NY[website] |
Gregory
B. Martin (co-PI) |
Cornell
University, Ithaca, NY [website] |
Xiaoyan
Tang (co-PI)
|
Kansas
State University, Manhattan, KS [website] |
Research
funded
by NSF-PGRP DBI-0077622
Lindeberg, M. et al, 2006. Closing the circle on the discovery of genes encoding Hrp regulon members and type III secretion system effectors in the genomes of three model Pseudomonas syringae strains. MPMI 19:1151-1158.
Schechter, L. M., et al. 2006. Multiple approaches to a complete inventory of Pseudomonas syringae pv tomato DC3000 type III secretion system effector proteins MPMI 19:1180-1192.
Vencato, M., et al., 2006. Bioinformatics-enabled identification of the HrpL regulon and type III secretion system effector proteins of Pseudomonas syringae pv. phaseolicola 1448A. MPMI 19:1193-1206.
Ferreira, A. et al.,2006. Whole-genome expression profiling defines the HrpL regulon of Pseudomonas syringae pv tomato DC3000 allows de novo reconstruction of the Hrp cis element, and identifies novel co-regulated genes. MPMI 19:1167-1179.
Oh, H. S. and Collmer, A. 2005. Basal resistance against bacteria in Nicotiana benthamiana leaves is accompanied by reduced vascular staining and suppressed by multiple Pseudomonas syringae type III secretion system effector proteins. Plant J. 44:348-59.
Joardar, V. et al. 2005. Whole-Genome Sequence Analysis of Pseudomonas syringae pv. phaseolicola 1448A Reveals Divergence among Pathovars in Genes Involved in Virulence and Transposition. J. Bacteriol 187:6488-6498.
Guo,
M., et al. 2005. Pseudomonas syringae type III chaperones
ShcO1, ShcS1, and ShcS2 facilitate translocation of their cognate
effectors and can substitute for each other in the secretion of
HopO1-1. J Bacteriol 187(12): 4257-69.
Lindeberg,
M., et al. 2005. Proposed guidelines for a unified nomenclature
and phylogenetic analysis of type III Hop effector proteins in the
plant pathogen Pseudomonas syringae. Mol. Plant Microbe Interact.
18: 275-282.
Joardar,
et al. 2005. Lineage-specific regions in Pseudomonas syringae
pv. tomato DC3000. Mol. Plant Path. 6:53 ( see also supplementary material
text, Table
S1, Table S2,
Table S3, Fig
S1)
Petnicki-Ocwieja,
T., et al. 2005. The hrpK operon of Pseudomonas syringae
pv. tomato DC3000 encodes two proteins secreted by the type
III (Hrp) protein secretion system: HopB1 and HrpK, a putative type
III translocator. J Bacteriol 187(2): 649-63.
Lin
and Martin, 2005. An avrPto/avrPtoB Mutant of Pseudomonas syringae
pv. tomato DC3000 does not elicit Pto-mediated resistance and is
less virulent on tomato. Mol Plant Microbe Interact. 18:43-51
Espinosa,
A. and Alfano, J. R. 2004. Disabling surveillance: bacterial
type III secretion system effectors that suppress innate immunity.
Cell Microbiol 6(11): 1027-40.
Lopez-Solanilla,
et al. 2004. HopPtoN is a Pseudomonas syringae Hrp (type
III secretion system) cysteine protease effector that suppresses
pathogen-induced necrosis associated with both compatible and incompatible
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Wehling,
M. D. et al. 2004. The Pseudomonas syringae HopPtoV Protein
Is Secreted in Culture and Translocated into Plant Cells via the
Type III Protein Secretion System in a Manner Dependent on the ShcV
Type III Chaperone. J. Bacteriol. 186: 3621-3630.
He,
P., et al. 2004. Activation of a COI1-dependent pathway in Arabidopsis
by Pseudomonas syringae type III effectors and coronatine.
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Jamir,
et al. 2004a. Identification of Pseudomonas syringae
type III effectors that can suppress programmed cell death in plants
and yeast. Plant J. 37:554-565.
Jamir, et al.
2004b. The genome of Pseudomonas syringae pv. tomato DC3000
and functional genomic studies to better understand plant pathogenesis.
Pages 113-138 in.: Pseudomonas, Volume 1: Genomics, LifeStyle
and Molecular Architecture. J. L. Ramos, ed. Kluwer/Plenum, London.
Shan,
et al. 2004. The HopPtoF locus of Pseudomonas syringae
pv. tomato DC3000 encodes a type III chaperone and cognate effector.
Mol. Plant-Microbe. Interact. 17:447-455
D'Ascenzo
et al. 2004. PeerGAD: a peer-review-based and community-centric
web application for viewing and annotating prokaryotic genome sequences.
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Schechter,
et al. 2004. Pseudomonas syringae Type III secretion
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Alfano
and Collmer, 2004. Type III secretion system effector proteins:
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Alfano and Guo,
2003. The Pseudomonas syringae Hrp (type III_ protein secretion
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Interactions, Vol 6. G. Stacey and N. T. Keen, ed. APS Press, St.
Paul.
Chatterjee,
et al. 2003. GacA, the response regulator of a two-component
system, acts as a master regulator in Pseudomonas syringae pv
tomato DC3000 by controlling regulatory RNA, transcriptional
activators, and alternate sigma factors. Mol. Plant Microbe Interact.
16:1106-1117.
Buell,
et al. 2003. The complete sequence of the Arabidopsis and tomato
pathogen Pseudomonas syringae pv. tomato DC3000. Proc.
Natl. Acad. Sci 100:10181-10186. (see also supporting
text, supporting
Table 2, Table 3,
Table 4, Table
5, Table 6, Fig
4, Fig 5, Fig
6)
Badel,
et al. 2003. Pseudomonas syringae pv tomato DC3000
HopPtoM (CEL :ORF3) is a major factor in tomato and is secreted
and translocated by the Hrp type III secretion system in a chaperone
dependent manner. Mol Microbiol. 49:1239-1251.
Espinosa,
2003. The Pseudomonas syringae type III-secreted protein
HopPtoD2 possesses protein tyrosine phosphatase activity and suppresses
programmed cell death in plants. Mol Microbiol. 49:377-387.
Deng,
W. L., et al. 2003. Pseudomonas syringae exchangeable
effector loci: sequence diversity in representative pathovars and
virulence function in P. syringae pv. syringae B728a. J Bacteriol
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Abramovitch,
2003. Pseudomonas type III effector AvrPtoB induces plant
disease susceptibility by inhibition of host programmed cell death
EMBO J. 22:60-69
Collmer,
et al. 2002. Genomic mining type III secretion system effectors
in Pseudomonas syringae yields new picks for all TTSS prospectors.
Trends in Microbiol. 10:462-469
Kim,
et al. 2002. Two distinct Pseudomonas effector proteins
interact with the Pto kinase and activate plant immunity. Cell
73: 589-598
Petnicki-Ocwieja,
et al 2002. Genomewide identification of proteins secreted by
the Hrp type III protein secretion system of Pseudomonas syringae
pv. tomato DC3000. Proc. Natl. Acad. Sci. 99:7652-7657
(see also supporting Table3,
Table4, text)
Fouts,
et al. 2002. Genomewide identification of Pseudomonas syringae
pv. tomato DC3000 promoters controlled by the HrpL alternative
sigma factor. Proc. Natl. Acad. Sci. 99:2275-2280. (see also supporting
info) |